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Short-term restraint and emotional-painful stressors increase DNA instability in different brain areas of rats with contrast excitability

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Abstract

Stress-reaction developed after exposure to stressors of different natures, increases the level of DNA damage in cells of target organs, including the central nervous system. However, the time of stressing exposure needed to induce genome destabilization in different brain areas and individual differences in animals defining their brain cell genome response to stressors is unclear. In this research, we show that acute stressors (2-h immobilization or 13-min emotional-painful stressor) increase the level of DNA damage in at least one of the brain regions studied: the prefrontal cortex, hippocampus, and amygdala in rat strains with the high or low threshold of nerve tibialis excitability, and non-selected Wistar rats. The results reveal the interstrain differences in the genome response to acute stressors of each brain area, different from the repeated emotional-painful stressor effects shown earlier.

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Data availability

Data reported in this study will be made available upon request from the corresponding author.

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Funding

The study was supported by the State funding allocated to the Pavlov Institute of Physiology Russian Academy of Sciences (№ 1021062411629–7-3.1.4).

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Authors and Affiliations

  1. Laboratory of Higher Nervous Activity Genetics, Pavlov Institute of Physiology, Russian Academy of Sciences, Nab. Makarova, 6, 199034, Saint Petersburg, Russia

    Veronika Shcherbinina, Eugene Daev, Marina Pavlova & Natalia Dyuzhikova

  2. Department of Genetics and Biotechnology, Faculty of Biology, Saint Petersburg State University, Universitetskaya Nab., 7–9, 199034, Saint Petersburg, Russia

    Veronika Shcherbinina & Eugene Daev

Authors
  1. Veronika Shcherbinina
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  2. Eugene Daev
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  3. Marina Pavlova
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  4. Natalia Dyuzhikova
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Contributions

Veronika Shcherbinina: Formal analysis, Investigation, Data Curation, Writing—Original Draft, Visualization. Eugene Daev: Conceptualization, Writing—Review & Editing, Supervision. Marina Pavlova: Methodology, Investigation, Writing—Review & Editing. Natalia Dyuzhikova: Conceptualization, Methodology, Resources, Writing—Review & Editing, Supervision, Project administration, Funding acquisition.

Corresponding author

Correspondence to Veronika Shcherbinina.

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Ethics

All animal experiments were conducted in accordance with the Council of the European community directives (86/609/EEC) on the use of animals for experimental research. The experimental protocol was approved by the Animal Care and Use Committee at the Pavlov Institute of Physiology of RAS (protocol No. 01/16 of 16 January 2023).

Competing interest

The authors have no competing interests to declare that are relevant to the content of this article.

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Shcherbinina, V., Daev, E., Pavlova, M. et al. Short-term restraint and emotional-painful stressors increase DNA instability in different brain areas of rats with contrast excitability. Neurosci Behav Physi (2024). https://doi.org/10.1007/s11055-024-01692-w

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  • DOI: https://doi.org/10.1007/s11055-024-01692-w

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